Hotspot

trnG-trnM

LSC · 40378-40705 · 328 bp

Back to Lophira

Pi 0.00915

MarkerSeek score 58.30

Primer available yes

Label trnG-trnM

Feature Metadata

label_name: trnG-trnM
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 3
pi: 0.00915
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnM_p1	TGCGGATATAGTCGAGTGGT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	433 bp	1.0	80.1
trnG-trnM_p2	TGCGGATATAGTCGAGTGGT	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.0	57.8	431 bp	1.0	78.9
trnG-trnM_p3	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	405 bp	1.0	78.9
trnG-trnM_p4	TGCGGATATAGTCGAGTGGT	GTAGCTCGCAAGGCTCATAA	50.0	50.0	58.0	57.8	454 bp	1.0	78.5
trnG-trnM_p5	TGCGGATATAGTCGAGTGGT	TAGCTCGCAAGGCTCATAAC	50.0	50.0	58.0	57.8	453 bp	1.0	78.5