Hotspot

atpF-atpH

LSC · 14775-15233 · 459 bp

Back to Lophira

Pi 0.00682

MarkerSeek score 63.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 3
pi: 0.00682
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCCCAAGGAAAGGAAAGAA	AGCGGAGGGAAAAATACGAG	50.0	50.0	58.0	57.7	592-611 bp	1.0	80.4
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGCGGAGGGAAAAATACGAG	50.0	50.0	57.9	57.7	624-643 bp	1.0	79.3
atpF-atpH_p3	GCCCCAAGGAAAGGAAAGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	590-609 bp	1.0	77.4
atpF-atpH_p4	GCCCCAAGGAAAGGAAAGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	591-610 bp	1.0	77.2
atpF-atpH_p5	GCCCCAAGGAAAGGAAAGAA	AAGCGGAGGGAAAAATACGA	50.0	45.0	58.0	57.2	593-612 bp	1.0	76.7