Hotspot

trnS-trnG

LSC · 9277-10423 · 1147 bp

Back to Lophira

Pi 0.00281

MarkerSeek score 64.70

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 3
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	CAATCCGACGCTTTAGTCCA	GAATCTGAGTCAAGGGATCCC	50.0	52.4	58.0	57.5	1199-1254 bp	1.0	72.3
trnS-trnG_p2	CAATCCGACGCTTTAGTCCA	GGAATCTGAGTCAAGGGATCC	50.0	52.4	58.0	57.5	1200-1255 bp	1.0	72.3
trnS-trnG_p3	AATCCGACGCTTTAGTCCAC	GAATCTGAGTCAAGGGATCCC	50.0	52.4	58.0	57.5	1198-1253 bp	1.0	72.3
trnS-trnG_p4	AATCCGACGCTTTAGTCCAC	GGAATCTGAGTCAAGGGATCC	50.0	52.4	58.0	57.5	1199-1254 bp	1.0	72.3
trnS-trnG_p5	ATCATCCATCAACGGAACCG	GAATCTGAGTCAAGGGATCCC	50.0	52.4	58.0	57.5	1270-1325 bp	1.0	71.9