Hotspot

ndhH-trnN-GUU

SSC · 124459-131867 · 7409 bp

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Pi 0.0453

MarkerSeek score 78.40

Primer available yes

Label ndhH-trnN-GUU

Feature Metadata

label_name: ndhH-trnN-GUU
feature_type: igs
region: SSC
length_bp: 7409
variable_sites: 1964
pi: 0.0453
unique_haplotype_count: 77
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-trnN-GUU_p1	GATACCCACGCCATCCTTTT	CGATTGGGACTTGAGACAGA	50.0	50.0	57.9	57.0	366-670 bp	0.2375	52.8
ndhH-trnN-GUU_p2	GATACCCACGCCATCCTTTT	TCGATTGGGACTTGAGACAG	50.0	50.0	57.9	57.0	367-671 bp	0.2375	52.8
ndhH-trnN-GUU_p3	TCCCAAGCGATCGATTTGAG	CGATTGGGACTTGAGACAGA	50.0	50.0	58.1	57.0	561-609 bp	0.2	51.2
ndhH-trnN-GUU_p4	TCCCAAGCGATCGATTTGAG	TCGATTGGGACTTGAGACAG	50.0	50.0	58.1	57.0	562-610 bp	0.2	51.2
ndhH-trnN-GUU_p5	GAACTTTTCGCGGAAGAACG	CGATTGGGACTTGAGACAGA	50.0	50.0	58.1	57.0	290-303 bp	0.125	48.0