Hotspot

ndhF-trnL-UAG

SSC · 114484-115550 · 1067 bp

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Pi 0.0207
MarkerSeek score 74.40
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1067
variable_sites
188
pi
0.0207
unique_haplotype_count
68
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TCTTGTATTTATCGTATGTATCCA AGCGTGTCTACCGATTTCAC 29.2 50.0 53.2 58.0 941-1199 bp 1.0 52.0
ndhF-trnL-UAG_p2 TCTTGTATTTATCGTATGTATCCA CAGCGTGTCTACCGATTTCA 29.2 50.0 53.2 58.0 942-1200 bp 1.0 52.0
ndhF-trnL-UAG_p3 TCTTGTATTTATCGTATGTATCCA TCCTAAGAGCAGCGTGTCTA 29.2 50.0 53.2 57.9 951-1209 bp 1.0 52.0
ndhF-trnL-UAG_p4 TCTTGTATTTATCGTATGTATCCA CCTAAGAGCAGCGTGTCTAC 29.2 55.0 53.2 57.8 950-1208 bp 1.0 52.0
ndhF-trnL-UAG_p5 TCTTGTATTTATCGTATGTATCCA CTAAGAGCAGCGTGTCTACC 29.2 55.0 53.2 57.8 949-1207 bp 1.0 52.0