Hotspot

trnN-GUU-ndhF

IRb · 111246-112221 · 976 bp

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Pi 0.152
MarkerSeek score 80.50
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
976
variable_sites
608
pi
0.152
unique_haplotype_count
72
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 CCGAACGCTCTACCACTAAG GCATATTGATATGTCTGTTCCA 55.0 36.4 57.8 54.1 861-1450 bp 0.925 49.0
trnN-GUU-ndhF_p2 CCCCAAGTAGGATTCGAACC GCATATTGATATGTCTGTTCCA 55.0 36.4 57.7 54.1 901-1490 bp 0.925 49.0
trnN-GUU-ndhF_p3 CGAACGCTCTACCACTAAGC GCATATTGATATGTCTGTTCCA 55.0 36.4 58.5 54.1 860-1449 bp 0.925 49.0
trnN-GUU-ndhF_p4 CCGAACGCTCTACCACTAAG GCATATTGATATGTCTGTTCCAT 55.0 34.8 57.8 54.6 861-1450 bp 0.925 49.0
trnN-GUU-ndhF_p5 CCCCAAGTAGGATTCGAACC GCATATTGATATGTCTGTTCCAT 55.0 34.8 57.7 54.6 901-1490 bp 0.925 49.0