Hotspot

infA-rps8

LSC · 82314-82493 · 180 bp

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Pi 0.0421

MarkerSeek score 72.20

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 48
pi: 0.0421
unique_haplotype_count: 47
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	TTGGACTCTATCGCCTGGAA	CGGGATGGGGGTTGTAATTC	50.0	55.0	58.1	58.3	376-407 bp	0.9	82.9
infA-rps8_p2	TTGGACTCTATCGCCTGGAA	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.1	58.4	305-336 bp	0.9	82.8
infA-rps8_p3	TTGGACTCTATCGCCTGGAA	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.1	57.6	304-335 bp	0.9	82.7
infA-rps8_p4	TTGGACTCTATCGCCTGGAA	AGAATTTTAGGCGGGATGGG	50.0	50.0	58.1	57.6	387-418 bp	0.9	82.4
infA-rps8_p5	TTGGACTCTATCGCCTGGAA	TCGACTAGAAAGAATCGGCG	50.0	50.0	58.1	57.5	319-350 bp	0.875	81.1