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rps12-psbB

LSC · 73544-74725 · 1182 bp

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Pi 0.0116

MarkerSeek score 68.90

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 1182
variable_sites: 125
pi: 0.0116
unique_haplotype_count: 56
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	GGGGGCTTTTGTGACATTTC	ATGGACAGAAAGCAGTCGAC	50.0	50.0	57.5	57.9	1294-1338 bp	1.0	85.8
rps12-psbB_p2	GGGGGCTTTTGTGACATTTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.5	58.2	1245-1289 bp	1.0	84.8
rps12-psbB_p3	GGGGGCTTTTGTGACATTTC	GTCGACCGGGATCATTCAAT	50.0	50.0	57.5	57.8	1280-1324 bp	0.9875	84.0
rps12-psbB_p4	GTACATGTTCCTCGACGCTG	ATGGACAGAAAGCAGTCGAC	55.0	50.0	58.7	57.9	1332-1376 bp	1.0	83.3
rps12-psbB_p5	AGTACATGTTCCTCGACGCT	ATGGACAGAAAGCAGTCGAC	50.0	50.0	58.8	57.9	1333-1377 bp	1.0	82.5