Hotspot

rbcL-accD

LSC · 58390-60723 · 2334 bp

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Pi 0.128

MarkerSeek score 76.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 2334
variable_sites: 1319
pi: 0.128
unique_haplotype_count: 77
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTTGCTCGTGACGGTAATGA	CCCCCATAAACCCAAACTCC	50.0	55.0	58.0	58.1	2158-2997 bp	0.8625	85.3
rbcL-accD_p2	TCTTGCTCGTGACGGTAATG	CCCCCATAAACCCAAACTCC	50.0	55.0	58.0	58.1	2159-2998 bp	0.8625	85.3
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	TACCAAAACTGGCTGTCACC	50.0	50.0	57.9	58.0	2341-2999 bp	0.475	69.9
rbcL-accD_p4	CTTGCTCGTGACGGTAATGA	CCACCGGTAGTAGGAGATGT	50.0	55.0	58.0	57.9	2381-2999 bp	0.4625	69.9
rbcL-accD_p5	TCTTGCTCGTGACGGTAATG	CCACCGGTAGTAGGAGATGT	50.0	55.0	58.0	57.9	2382-3000 bp	0.4625	69.9