Hotspot

atpH-atpI

LSC · 14484-15635 · 1152 bp

Back to Lonicera

Pi 0.0098

MarkerSeek score 73.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1152
variable_sites: 133
pi: 0.0098
unique_haplotype_count: 68
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1235-1273 bp	1.0	87.6
atpH-atpI_p2	GTTCCTTGACCAACTCCAGG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1292-1330 bp	1.0	87.0
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	1208-1246 bp	1.0	87.0
atpH-atpI_p4	CAGCAGTTCCTTGACCAACT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	1297-1335 bp	0.95	85.5
atpH-atpI_p5	CAGCAGTTCCTTGACCAACT	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.0	57.4	1270-1308 bp	0.95	85.1