Hotspot

trnS-GCU-trnS-CGA

LSC · 9090-9834 · 745 bp

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Pi 0.0138

MarkerSeek score 68.90

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 117
pi: 0.0138
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	45.0	40.9	58.0	57.7	827-892 bp	0.9875	72.8
trnS-GCU-trnS-CGA_p2	GCTCGTACAACGGATTAGCA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.1	57.7	840-905 bp	0.9875	72.4
trnS-GCU-trnS-CGA_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.2	57.7	876-941 bp	0.9875	71.9
trnS-GCU-trnS-CGA_p4	AGCTCGTACAACGGATTAGC	TGAATCAAACCGAAAGATCCCT	50.0	40.9	57.8	57.7	841-906 bp	0.9875	71.4
trnS-GCU-trnS-CGA_p5	TGGAAACGGAAAGAGAGGGA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.3	57.7	881-946 bp	0.9875	70.9