Hotspot

ndhI

SSC · 116537-117040 · 504 bp

Back to Lomatogonium

Pi 0.443

MarkerSeek score 77.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 179
pi: 0.443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TTTTGGGGGTGGAATCTGTG	CAATCCCAGCGAAAAAGCAG	50.0	50.0	58.0	58.0	1584 bp	0.5	59.8
ndhI_p2	TTTTGGGGGTGGAATCTGTG	TGACCCCTAAACCCCCTAAG	50.0	55.0	58.0	58.0	1540 bp	0.5	59.7
ndhI_p3	GGTTTTTGGGGGTGGAATCT	CAATCCCAGCGAAAAAGCAG	50.0	50.0	58.0	58.0	1587 bp	0.5	59.7
ndhI_p4	CTCTAGCCGAGTGTGAGAGA	CAATCCCAGCGAAAAAGCAG	55.0	50.0	58.0	58.0	1526 bp	0.5	59.6
ndhI_p5	GGTTTTTGGGGGTGGAATCT	TGACCCCTAAACCCCCTAAG	50.0	55.0	58.0	58.0	1543 bp	0.5	59.6