Hotspot

ndhH

SSC · 113013-114194 · 1182 bp

Back to Lomatogonium

Pi 0.478

MarkerSeek score 80.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 472
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AAAAGATTTGTGCGTGTGCC	ATTTCACCGAATCGCACGTA	45.0	45.0	58.1	58.0	2456 bp	0.5	58.8
ndhH_p2	AAAAGATTTGTGCGTGTGCC	TATTTCACCGAATCGCACGT	45.0	45.0	58.1	58.0	2457 bp	0.5	58.8
ndhH_p3	AAAAGATTTGTGCGTGTGCC	ACAAGGTGGTAGCCAAAAGG	45.0	50.0	58.1	58.0	2254 bp	0.5	58.8
ndhH_p4	CTCAGAGAGGGTTGCGAAAA	ATTTCACCGAATCGCACGTA	50.0	45.0	57.8	58.0	2014 bp	0.5	58.6
ndhH_p5	CTCAGAGAGGGTTGCGAAAA	TATTTCACCGAATCGCACGT	50.0	45.0	57.8	58.0	2015 bp	0.5	58.6