Hotspot

ndhG

SSC · 117436-117966 · 531 bp

Back to Lomatogonium

Pi 0.492

MarkerSeek score 79.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 220
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GTGTCGAGTATTGCCCAACA	TTGCTGAAACAATAGCCGGT	50.0	45.0	58.2	58.1	1470 bp	0.5	57.7
ndhG_p2	TGTGTCGAGTATTGCCCAAC	TTGCTGAAACAATAGCCGGT	50.0	45.0	58.2	58.1	1471 bp	0.5	57.7
ndhG_p3	GTGTCGAGTATTGCCCAACA	CTGAAACAATAGCCGGTCCA	50.0	50.0	58.2	57.9	1467 bp	0.5	57.6
ndhG_p4	TGTGTCGAGTATTGCCCAAC	CTGAAACAATAGCCGGTCCA	50.0	50.0	58.2	57.9	1468 bp	0.5	57.6
ndhG_p5	GTGTCGAGTATTGCCCAACA	ACAATAGCCGGTCCAATAGC	50.0	50.0	58.2	57.7	1462 bp	0.5	56.4