Hotspot

ndhE

SSC · 118088-118390 · 303 bp

Back to Lomatogonium

Pi 0.47

MarkerSeek score 75.70

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 127
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CTGCTTTTTCGCTGGGATTG	CCAGAAAAACCAGTGCTCCT	50.0	50.0	58.0	58.0	1519 bp	0.5	59.8
ndhE_p2	CTTAGGGGGTTTAGGGGTCA	CCAGAAAAACCAGTGCTCCT	55.0	50.0	58.0	58.0	1563 bp	0.5	59.7
ndhE_p3	CTGCTTTTTCGCTGGGATTG	AACCAACGCAGTCTTCTGTT	50.0	45.0	58.0	58.0	1328 bp	0.5	59.6
ndhE_p4	CCCGAACCAATACCGGATTT	CCAGAAAAACCAGTGCTCCT	50.0	50.0	58.0	58.0	1613 bp	0.5	59.5
ndhE_p5	CTTAGGGGGTTTAGGGGTCA	AACCAACGCAGTCTTCTGTT	55.0	45.0	58.0	58.0	1372 bp	0.5	59.5