Hotspot

ndhD

SSC · 119432-120595 · 1164 bp

Back to Lomatogonium

Pi 0.476

MarkerSeek score 81.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1164
variable_sites: 483
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGAGCACTGGTTTTTCTGG	GTGAACCGACACGAATCGTA	50.0	50.0	58.0	58.0	1720 bp	0.5	59.6
ndhD_p2	AACAGAAGACTGCGTTGGTT	GTGAACCGACACGAATCGTA	45.0	50.0	58.0	58.0	1911 bp	0.5	59.4
ndhD_p3	AGCTACTTTATCGGCTTGGC	GTGAACCGACACGAATCGTA	50.0	50.0	58.0	58.0	1381 bp	0.5	59.4
ndhD_p4	AACAAATCGCTTCTGCTCCA	GTGAACCGACACGAATCGTA	45.0	50.0	58.1	58.0	1933 bp	0.5	58.9
ndhD_p5	ACAAATCGCTTCTGCTCCAA	GTGAACCGACACGAATCGTA	45.0	50.0	58.1	58.0	1932 bp	0.5	58.9