Hotspot

ndhG-ndhE

SSC · 117967-118087 · 121 bp

Back to Lomatogonium

Pi 0.458

MarkerSeek score 75.50

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 121
variable_sites: 49
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	CTGCTTTTTCGCTGGGATTG	TTGCTGAAACAATAGCCGGT	50.0	45.0	58.0	58.1	796 bp	0.5	59.1
ndhG-ndhE_p2	CTTAGGGGGTTTAGGGGTCA	TTGCTGAAACAATAGCCGGT	55.0	45.0	58.0	58.1	840 bp	0.5	59.0
ndhG-ndhE_p3	CTGCTTTTTCGCTGGGATTG	CTGAAACAATAGCCGGTCCA	50.0	50.0	58.0	57.9	793 bp	0.5	59.0
ndhG-ndhE_p4	CTGCTTTTTCGCTGGGATTG	TGCTTTACATCCGTCCCAAG	50.0	50.0	58.0	57.9	1285 bp	0.5	59.0
ndhG-ndhE_p5	CTTAGGGGGTTTAGGGGTCA	CTGAAACAATAGCCGGTCCA	55.0	50.0	58.0	57.9	837 bp	0.5	58.9