Hotspot

ndhA-ndhI

SSC · 116461-116536 · 76 bp

Back to Lomatogonium

Pi 0.485

MarkerSeek score 75.80

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: SSC
length_bp: 76
variable_sites: 33
pi: 0.485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	TTTTGGGGGTGGAATCTGTG	GTTGGGCAATACTCGACACA	50.0	50.0	58.0	58.2	909 bp	0.5	58.4
ndhA-ndhI_p2	TTTTGGGGGTGGAATCTGTG	TGTTGGGCAATACTCGACAC	50.0	50.0	58.0	58.2	910 bp	0.5	58.4
ndhA-ndhI_p3	GGTTTTTGGGGGTGGAATCT	GTTGGGCAATACTCGACACA	50.0	50.0	58.0	58.2	912 bp	0.5	58.2
ndhA-ndhI_p4	GGTTTTTGGGGGTGGAATCT	TGTTGGGCAATACTCGACAC	50.0	50.0	58.0	58.2	913 bp	0.5	58.2
ndhA-ndhI_p5	CTCTAGCCGAGTGTGAGAGA	GTTGGGCAATACTCGACACA	55.0	50.0	58.0	58.2	851 bp	0.5	58.2