Hotspot

rps15-ndhH

SSC · 112883-113012 · 130 bp

Back to Lomatogonium

Pi 0.398

MarkerSeek score 77.20

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 130
variable_sites: 49
pi: 0.398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	GCTGAAACGTTGTTGGTAGC	TCCCAACGCGTTACATAAGG	50.0	50.0	58.0	58.0	1001 bp	0.5	59.6
rps15-ndhH_p2	GCTGAAACGTTGTTGGTAGC	CAGAAGCTCGTAGCATTGGT	50.0	50.0	58.0	58.0	1471 bp	0.5	59.6
rps15-ndhH_p3	GCTGAAACGTTGTTGGTAGC	GATGAGCCGAAGAACACCAT	50.0	50.0	58.0	58.0	876 bp	0.5	59.5
rps15-ndhH_p4	GCTGAAACGTTGTTGGTAGC	CCGAGCCATAACAAATGGGA	50.0	50.0	58.0	57.9	1145 bp	0.5	59.3
rps15-ndhH_p5	GCTGAAACGTTGTTGGTAGC	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	864 bp	0.5	59.3