Hotspot

psbA

LSC · 87-1148 · 1062 bp

Back to Lolium

Pi 0
MarkerSeek score 28.10
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TCCTATATGTTAGCAGAAAAACC TATGTGTGCTTGGGAGTCCT 34.8 50.0 54.2 58.3 1133 bp 1.0 40.0
psbA_p2 TTCCTATATGTTAGCAGAAAAACC TATGTGTGCTTGGGAGTCCT 33.3 50.0 54.9 58.3 1134 bp 1.0 40.0
psbA_p3 TCCTATATGTTAGCAGAAAAACC TGGGAGTCCTTGCAATTTGA 34.8 45.0 54.2 57.3 1123 bp 1.0 40.0
psbA_p4 TCCTATATGTTAGCAGAAAAACC ATATGTGTGCTTGGGAGTCC 34.8 50.0 54.2 57.3 1134 bp 1.0 40.0
psbA_p5 TCCTATATGTTAGCAGAAAAACC GCTTGGGAGTCCTTGCAATT 34.8 50.0 54.2 58.7 1126 bp 1.0 40.0