Hotspot

ndhI

SSC · 110003-110545 · 543 bp

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Pi 0.00368
MarkerSeek score 59.70
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
543
variable_sites
2
pi
0.00368
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 AGGATTCCAGGAAGTAAAAAGGG TCAACACAAAGGCTCTGTCA 43.5 45.0 58.1 57.3 665 bp 1.0 50.1
ndhI_p2 GGATTCCAGGAAGTAAAAAGGGA TCAACACAAAGGCTCTGTCA 43.5 45.0 58.1 57.3 664 bp 1.0 50.0
ndhI_p3 GGATTCCAGGAAGTAAAAAGGG TCAACACAAAGGCTCTGTCA 45.5 45.0 56.7 57.3 664 bp 1.0 47.8
ndhI_p4 AGGATTCCAGGAAGTAAAAAGGG TCAACACAAAGGCTCTGTCAA 43.5 42.9 58.1 58.0 665 bp 1.0 47.7
ndhI_p5 AGGATTCCAGGAAGTAAAAAGGG TTCAACACAAAGGCTCTGTCA 43.5 42.9 58.1 58.0 666 bp 1.0 47.7