Hotspot

ndhF-rpl32

SSC · 103597-104366 · 770 bp

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Pi 0.00427
MarkerSeek score 62.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
770
variable_sites
3
pi
0.00427
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGCTAAAGAATAAGCCTGTACT 55.0 36.4 58.1 54.9 857-925 bp 1.0 44.3
ndhF-rpl32_p2 GGCCAAACCCCATTGACATA TGCTAAAGAATAAGCCTGTACT 50.0 36.4 58.1 54.9 883-951 bp 1.0 44.3
ndhF-rpl32_p3 AAAAGGCCAAACCCCATTGA TGCTAAAGAATAAGCCTGTACT 45.0 36.4 58.2 54.9 887-955 bp 1.0 44.3
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTGCTAAAGAATAAGCCTGTACT 55.0 34.8 58.1 55.7 858-926 bp 1.0 44.3
ndhF-rpl32_p5 GGCCAAACCCCATTGACATA TTGCTAAAGAATAAGCCTGTACT 50.0 34.8 58.1 55.7 884-952 bp 1.0 44.3