Hotspot

rps11-rpl36

LSC · 75271-75462 · 192 bp

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Pi 0.0156
MarkerSeek score 48.50
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
192
variable_sites
3
pi
0.0156
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 GCGCACTCTTCCGTAAACTA AGCATAAACAAAGACAGGGGT 50.0 42.9 58.0 57.5 306-307 bp 1.0 68.5
rps11-rpl36_p2 TATTCTACGCGCACTCTTCC AGCATAAACAAAGACAGGGGT 50.0 42.9 57.5 57.5 314-315 bp 1.0 64.7
rps11-rpl36_p3 GCGCACTCTTCCGTAAACTA GCATAAACAAAGACAGGGGT 50.0 45.0 58.0 55.9 305-306 bp 1.0 64.0
rps11-rpl36_p4 GCGCACTCTTCCGTAAACTA CCGAAGCATAAACAAAGACAGG 50.0 45.5 58.0 57.9 310-311 bp 1.0 64.0
rps11-rpl36_p5 GCGCACTCTTCCGTAAACTA CGAAGCATAAACAAAGACAGGG 50.0 45.5 58.0 57.9 309-310 bp 1.0 64.0