Hotspot

petA-psbJ

LSC · 60169-60956 · 788 bp

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Pi 0.00254
MarkerSeek score 60.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
788
variable_sites
2
pi
0.00254
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC ACGGTTCATATTCTGGATTGGG 50.0 45.5 57.9 58.2 925-932 bp 1.0 62.3
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC CGGTTCATATTCTGGATTGGGT 50.0 45.5 57.9 58.2 924-931 bp 1.0 62.3
petA-psbJ_p3 TGTTCTTCTTCGCATCCGTT ACGGTTCATATTCTGGATTGGG 45.0 45.5 57.8 58.2 918-925 bp 1.0 61.4
petA-psbJ_p4 TGTTCTTCTTCGCATCCGTT CGGTTCATATTCTGGATTGGGT 45.0 45.5 57.8 58.2 917-924 bp 1.0 61.4
petA-psbJ_p5 TTGTTCTTCTTCGCATCCGT CGGTTCATATTCTGGATTGGGT 45.0 45.5 57.8 58.2 918-925 bp 1.0 61.4