Hotspot

rbcL-psaI

LSC · 55673-56854 · 1182 bp

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Pi 0.0135
MarkerSeek score 66.50
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1182
variable_sites
16
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 AGTTCGAGCCGGTAGATACT CCATTGCAATTGCCGGAAAT 50.0 45.0 57.7 58.0 1284-1285 bp 1.0 78.0
rbcL-psaI_p2 GGCGATCAAATTCGAGTTCG CCATTGCAATTGCCGGAAAT 50.0 45.0 57.6 58.0 1298-1299 bp 1.0 77.9
rbcL-psaI_p3 TGGAAGGCGATCAAATTCGA CCATTGCAATTGCCGGAAAT 45.0 45.0 57.6 58.0 1303-1304 bp 1.0 77.6
rbcL-psaI_p4 AGTTCGAGCCGGTAGATACT AAGAAGCCATTGCAATTGCC 50.0 45.0 57.7 57.9 1290-1291 bp 1.0 77.4
rbcL-psaI_p5 GGCGATCAAATTCGAGTTCG AAGAAGCCATTGCAATTGCC 50.0 45.0 57.6 57.9 1304-1305 bp 1.0 77.3