Hotspot

atpB-rbcL

LSC · 53446-54238 · 793 bp

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Pi 0.00126
MarkerSeek score 59.50
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
793
variable_sites
1
pi
0.00126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AAAGTGACATCTAGCACGGG CCTTGGTTTCATACTCCGGG 50.0 55.0 57.6 58.0 994 bp 1.0 76.7
atpB-rbcL_p2 AAAGTGACATCTAGCACGGG CTTGGTTTCATACTCCGGGG 50.0 55.0 57.6 58.0 993 bp 1.0 76.7
atpB-rbcL_p3 AAAGTGACATCTAGCACGGG ACTCCGGGGTGTAGTAAGTC 50.0 55.0 57.6 58.2 982 bp 1.0 75.7
atpB-rbcL_p4 AAAGTGACATCTAGCACGGG CTCCGGGGTGTAGTAAGTCA 50.0 55.0 57.6 58.2 981 bp 1.0 75.7
atpB-rbcL_p5 AAAGTGACATCTAGCACGGG TCCGGGGTGTAGTAAGTCAA 50.0 50.0 57.6 57.7 980 bp 1.0 74.5