Hotspot

ndhC-trnV-UAC

LSC · 49593-50486 · 894 bp

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Pi 0.00237
MarkerSeek score 64.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
894
variable_sites
2
pi
0.00237
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCTGAAATCCAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.0 57.9 953-1004 bp 1.0 83.3
ndhC-trnV-UAC_p2 GCCCTGAAATCCAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.0 58.3 954-1005 bp 1.0 81.5
ndhC-trnV-UAC_p3 CTCAGGTCCTTCACTAACCG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.3 57.9 982-1033 bp 1.0 78.3
ndhC-trnV-UAC_p4 TCAGGTCCTTCACTAACCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.7 57.9 981-1032 bp 1.0 77.7
ndhC-trnV-UAC_p5 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 972-1023 bp 1.0 77.4