Hotspot

ycf3-trnS-GGA

LSC · 43519-44114 · 596 bp

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Pi 0.00336
MarkerSeek score 59.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
596
variable_sites
2
pi
0.00336
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTCAA 50.0 45.0 58.1 57.8 729 bp 1.0 78.2
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT AGTTCCAATGCTACGCCTTC 50.0 50.0 58.1 58.3 731 bp 1.0 77.6
ycf3-trnS-GGA_p3 TTCTCCTGAGGTTGTCGGAA TTCCAATGCTACGCCTTCAA 50.0 45.0 58.3 57.8 730 bp 1.0 76.4
ycf3-trnS-GGA_p4 TTTCTCCTGAGGTTGTCGGA TTCCAATGCTACGCCTTCAA 50.0 45.0 58.3 57.8 731 bp 1.0 76.4
ycf3-trnS-GGA_p5 TGAAATTTCCATTTGCGCGG TTCCAATGCTACGCCTTCAA 45.0 45.0 58.3 57.8 665 bp 1.0 76.4