Hotspot

ycf1

SSC · 126731-132421 · 5691 bp

Back to Loeseneriella

Pi 0.00587

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5691
variable_sites: 33
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGAAATGGGCGATTCCATC	TCGATGAAACGAAAGAGAAA	50.0	35.0	58.4	52.9	230-236 bp	1.0	47.8
ycf1_p2	TCGAAATGGGCGATTCCATC	TCGATGAAACGAAAGAGAAAA	50.0	33.3	58.4	53.8	230-236 bp	1.0	47.8
ycf1_p3	TCGAAATGGGCGATTCCATC	ATCGATGAAACGAAAGAGAA	50.0	35.0	58.4	52.7	231-237 bp	1.0	47.8
ycf1_p4	TCGAAATGGGCGATTCCATC	TCGATGAAACGAAAGAGAAAAA	50.0	31.8	58.4	54.7	230-236 bp	1.0	47.8
ycf1_p5	TCGAAATGGGCGATTCCATC	AATCGATGAAACGAAAGAGA	50.0	35.0	58.4	52.7	232-238 bp	1.0	47.7