Hotspot

psbZ-trnG-GCC

LSC · 37549-38248 · 700 bp

Back to Loeseneriella

Pi 0.00875

MarkerSeek score 61.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 6
pi: 0.00875
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	858-961 bp	1.0	78.2
psbZ-trnG-GCC_p2	TCTCCGGGACATCCTTATGG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	815-918 bp	1.0	78.2
psbZ-trnG-GCC_p3	TGAGGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	847-950 bp	1.0	78.1
psbZ-trnG-GCC_p4	CTGAGGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.6	57.8	848-951 bp	1.0	77.5
psbZ-trnG-GCC_p5	TGTATTCTCCGGGACATCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.5	57.8	820-923 bp	1.0	76.7