Hotspot

atpH-atpI

LSC · 13637-14900 · 1264 bp

Back to Loeseneriella

Pi 0.00725

MarkerSeek score 64.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1264
variable_sites: 9
pi: 0.00725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	1349-1406 bp	1.0	80.0
atpH-atpI_p2	CAGCAGCAATAACAGAAGCG	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.8	57.9	1359-1416 bp	1.0	79.4
atpH-atpI_p3	AATAGAAGCAAGACCGACGG	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.7	57.9	1385-1442 bp	1.0	78.4
atpH-atpI_p4	CCGGGTCCAATAGAAGCAAG	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.3	57.9	1393-1450 bp	1.0	78.1
atpH-atpI_p5	ATGCCTTCTACAGCTTGACC	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.6	57.9	1435-1492 bp	1.0	77.4