Hotspot

ycf1

SSC · 121974-127298 · 5325 bp

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Pi 0.0116

MarkerSeek score 50.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5325
variable_sites: 62
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTTGATAAAGCTGATTCCAG	TGAAACCGTGGGTAATACCA	38.1	45.0	52.6	56.5	224 bp	1.0	42.5
ycf1_p2	CTTTGATAAAGCTGATTCCAG	TGAAACCGTGGGTAATACCAA	38.1	42.9	52.6	57.2	224 bp	1.0	42.5
ycf1_p3	CTTTGATAAAGCTGATTCCAG	ATGAAACCGTGGGTAATACCA	38.1	42.9	52.6	57.0	225 bp	1.0	42.5
ycf1_p4	CTTTGATAAAGCTGATTCCAG	ATGAAACCGTGGGTAATACCAA	38.1	40.9	52.6	57.7	225 bp	1.0	42.5
ycf1_p5	CTTTGATAAAGCTGATTCCAG	TGAAACCGTGGGTAATACCAAT	38.1	40.9	52.6	57.7	224 bp	1.0	42.5