Hotspot

trnF-GAA

LSC · 46373-46445 · 73 bp

Back to Lobularia

Pi 0.0411

MarkerSeek score 63.00

Primer available yes

Label trnF-GAA

Feature Metadata

label_name: trnF-GAA
feature_type: tRNA
region: LSC
length_bp: 73
variable_sites: 3
pi: 0.0411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA_p1	AATGAGGATGATACTTCGGT	ACGGTTAATTTGAAGAATAGAAACT	40.0	28.0	53.5	55.3	143 bp	0.5	20.0
trnF-GAA_p2	AAATGAGGATGATACTTCGGT	ACGGTTAATTTGAAGAATAGAAACT	38.1	28.0	54.4	55.3	144 bp	0.5	20.0
trnF-GAA_p3	AATGAGGATGATACTTCGGT	ACGGTTAATTTGAAGAATAGAAACTT	40.0	26.9	53.5	55.9	143 bp	0.5	20.0
trnF-GAA_p4	AATGAGGATGATACTTCGGT	ACGGTTAATTTGAAGAATAGAAAC	40.0	29.2	53.5	53.9	143 bp	0.5	20.0
trnF-GAA_p5	TGAGGATGATACTTCGGTAATG	ACGGTTAATTTGAAGAATAGAAACT	40.9	28.0	55.2	55.3	141 bp	0.5	20.0