Hotspot

petD

LSC · 74661-75844 · 1184 bp

Back to Lobularia

Pi 0.0101

MarkerSeek score 50.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1184
variable_sites: 12
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGGTGTATTGACCGCATCTT	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.0	57.9	2191-2192 bp	1.0	80.1
petD_p2	CCGTCCAACTGTTACTGAGG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	57.9	57.9	2389-2390 bp	1.0	79.7
petD_p3	GGGTTACTGGTGTGGTTCTG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	58.1	57.9	2210-2211 bp	1.0	79.6
petD_p4	GGTTACTGGTGTGGTTCTGG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	58.1	57.9	2209-2210 bp	1.0	79.6
petD_p5	GTTACTGGTGTGGTTCTGGG	GAAGCCTCCCGGAATTTGAT	55.0	50.0	58.1	57.9	2208-2209 bp	1.0	79.6