Hotspot

ndhC-trnV-UAC

LSC · 48698-49401 · 704 bp

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Pi 0.0229

MarkerSeek score 65.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 16
pi: 0.0229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGCCGGAAATTAGAAATGCC	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	810-813 bp	1.0	75.6
ndhC-trnV-UAC_p2	CGCCGGAAATTAGAAATGCC	TTCGAGTCCGTATAGCCCTA	50.0	50.0	57.9	57.1	809-812 bp	1.0	73.6
ndhC-trnV-UAC_p3	TGGAGATAAAACGCCGGAAA	GTTCGAGTCCGTATAGCCCT	45.0	55.0	57.5	58.7	821-824 bp	1.0	72.8
ndhC-trnV-UAC_p4	TTGGAGATAAAACGCCGGAA	GTTCGAGTCCGTATAGCCCT	45.0	55.0	57.5	58.7	822-825 bp	1.0	72.8
ndhC-trnV-UAC_p5	CGCCGGAAATTAGAAATGCC	TCGAACTCTTTTACCGAGCAG	50.0	47.6	57.9	58.1	838-841 bp	1.0	72.6