Hotspot

trnH-GUG-psbA

LSC · 75-364 · 290 bp

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Pi 0.0208
MarkerSeek score 65.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
290
variable_sites
6
pi
0.0208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT TGTTGAGGCTCCATCTACCA 50.0 50.0 57.9 58.0 344-346 bp 1.0 80.4
trnH-GUG-psbA_p2 CTGCCTTAATCCACTTGGCT GCTGTTGAGGCTCCATCTAC 50.0 55.0 57.9 58.1 346-348 bp 1.0 80.3
trnH-GUG-psbA_p3 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 361-363 bp 1.0 80.2
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC TGTTGAGGCTCCATCTACCA 50.0 50.0 58.2 58.0 345-347 bp 1.0 80.2
trnH-GUG-psbA_p5 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 364-366 bp 1.0 80.1