Hotspot

ORF198

LSC · 11665-12261 · 597 bp

Back to Lobelia

Pi 0.157

MarkerSeek score 80.30

Primer available yes

Label ORF198

Feature Metadata

label_name: ORF198
feature_type: gene
region: LSC
length_bp: 597
variable_sites: 483
pi: 0.157
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF198_p1	TATCAAAGCGCATCCAACGA	TTGTCGCTAGAGATTTGGGC	45.0	50.0	58.0	58.0	1664-2387 bp	0.533333	73.0
ORF198_p2	AGCGAACATCAATTGCAACG	TTGTCGCTAGAGATTTGGGC	45.0	50.0	58.0	58.0	1177-1821 bp	0.511111	72.0
ORF198_p3	CGCATCCAACGAACTCCTTA	TTGTCGCTAGAGATTTGGGC	50.0	50.0	58.0	58.0	1656-2379 bp	0.488889	71.3
ORF198_p4	CGCATCCAACGAACTCCTTA	TGACACGGGCAGTTGTAAAA	50.0	45.0	58.0	58.0	1572-1622 bp	0.244444	54.7
ORF198_p5	TATCAAAGCGCATCCAACGA	TGACACGGGCAGTTGTAAAA	45.0	45.0	58.0	58.0	1580-1630 bp	0.244444	54.6