Hotspot

trnK (UUU)-rps16

LSC · 10738-11224 · 487 bp

Back to Lobelia

Pi 0.0398
MarkerSeek score 75.70
Primer available yes
Label trnK (UUU)-rps16

Feature Metadata

label_name
trnK (UUU)-rps16
feature_type
igs
region
LSC
length_bp
487
variable_sites
192
pi
0.0398
unique_haplotype_count
36
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK (UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT AACGAACTTTGCCCCAATCA 50.0 45.0 58.2 58.3 548-672 bp 0.866667 82.9
trnK (UUU)-rps16_p2 AAAGCCGAGTACTCTACCGT ACGAACTTTGCCCCAATCAA 50.0 45.0 58.2 58.3 547-671 bp 0.866667 82.9
trnK (UUU)-rps16_p3 AAGCCGAGTACTCTACCGTT AACGAACTTTGCCCCAATCA 50.0 45.0 58.2 58.3 547-671 bp 0.866667 82.9
trnK (UUU)-rps16_p4 AAGCCGAGTACTCTACCGTT ACGAACTTTGCCCCAATCAA 50.0 45.0 58.2 58.3 546-670 bp 0.866667 82.9
trnK (UUU)-rps16_p5 TCAGTCGTGGTCTTCCAAAC ACGAACTTTGCCCCAATCAA 50.0 45.0 57.8 58.3 632-780 bp 0.866667 82.4