Hotspot

psbA-rbcL

LSC · 1481-2512 · 1032 bp

Back to Lobelia

Pi 0.0314

MarkerSeek score 87.40

Primer available yes

Label psbA-rbcL

Feature Metadata

label_name: psbA-rbcL
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 338
pi: 0.0314
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-rbcL_p1	TACAGAAGCGACCCCATAGG	ATTTGAGTTTGCCGCAATGG	55.0	45.0	58.6	57.9	862-1177 bp	1.0	86.6
psbA-rbcL_p2	TACAGAAGCGACCCCATAGG	GCTGCTTGTGAGGTATGGAA	55.0	50.0	58.6	57.9	891-1206 bp	1.0	86.4
psbA-rbcL_p3	TACAGAAGCGACCCCATAGG	TGAGTTTGCCGCAATGGATA	55.0	45.0	58.6	57.9	859-1174 bp	1.0	86.3
psbA-rbcL_p4	TACAGAAGCGACCCCATAGG	AGTTTGCCGCAATGGATACT	55.0	45.0	58.6	57.9	857-1172 bp	1.0	86.2
psbA-rbcL_p5	ATCCAGTTACAGAAGCGACC	ATTTGAGTTTGCCGCAATGG	50.0	45.0	57.3	57.9	869-1184 bp	1.0	85.9