Hotspot

trnH (GUG)-psbA

LSC · 78-418 · 341 bp

Back to Lobelia

Pi 0.0432
MarkerSeek score 83.50
Primer available yes
Label trnH (GUG)-psbA

Feature Metadata

label_name
trnH (GUG)-psbA
feature_type
igs
region
LSC
length_bp
341
variable_sites
143
pi
0.0432
unique_haplotype_count
34
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH (GUG)-psbA_p1 CAATCCACTGCCTTCATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 313-525 bp 1.0 88.2
trnH (GUG)-psbA_p2 AATCCACTGCCTTCATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 312-524 bp 1.0 88.2
trnH (GUG)-psbA_p3 CAATCCACTGCCTTCATCCA ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 314-526 bp 1.0 87.8
trnH (GUG)-psbA_p4 AATCCACTGCCTTCATCCAC ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 313-525 bp 1.0 87.7
trnH (GUG)-psbA_p5 ACAATCCACTGCCTTCATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 314-526 bp 0.977778 87.3