Hotspot

rps3

LSC · 79641-80360 · 720 bp

Back to Littledalea

Pi 0.00417

MarkerSeek score 47.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 6
pi: 0.00417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCTTTTGCAGGGATTTCAGGA	TCCATCTAAGATTTCGATTCCCA	42.9	39.1	57.5	57.1	849 bp	1.0	40.6
rps3_p2	GGACAGAATCCGAAGACAAGA	TCCATCTAAGATTTCGATTCCCA	47.6	39.1	57.4	57.1	795 bp	1.0	40.6
rps3_p3	TCTTTTGCAGGGATTTCAGGA	TCCATCTAAGATTTCGATTCCCAG	42.9	41.7	57.5	58.1	849 bp	1.0	40.6
rps3_p4	TCTTTTGCAGGGATTTCAGGA	CCATCTAAGATTTCGATTCCCAG	42.9	43.5	57.5	56.8	848 bp	1.0	40.6
rps3_p5	GGACAGAATCCGAAGACAAGA	TCCATCTAAGATTTCGATTCCCAG	47.6	41.7	57.4	58.1	795 bp	1.0	40.6