Hotspot

petB

LSC · 71632-73029 · 1398 bp

Back to Littledalea

Pi 0.000715

MarkerSeek score 49.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1398
variable_sites: 2
pi: 0.000715
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TAGTTCGACCGCGGAATTTT	ACCACAACCTACCCGATATGA	45.0	47.6	57.9	58.2	1497 bp	1.0	69.5
petB_p2	ATTATGGTAGTTCGACCGCG	ACCACAACCTACCCGATATGA	50.0	47.6	57.9	58.2	1504 bp	1.0	69.1
petB_p3	TAGTTCGACCGCGGAATTTT	ACCACAACCTACCCGATATG	45.0	50.0	57.9	56.7	1497 bp	1.0	69.1
petB_p4	TAGTTCGACCGCGGAATTTT	TGAAATACCACAACCTACCCG	45.0	47.6	57.9	57.7	1503 bp	1.0	68.7
petB_p5	ATTATGGTAGTTCGACCGCG	ACCACAACCTACCCGATATG	50.0	50.0	57.9	56.7	1504 bp	1.0	68.7