Hotspot

clpP-psbB

LSC · 68656-69190 · 535 bp

Back to Littledalea

Pi 0.0121
MarkerSeek score 51.80
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
535
variable_sites
13
pi
0.0121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 GTCGCTTCTTCATCTCCAGG CCAGCCCAACCAGAAACTAG 55.0 55.0 58.1 58.2 695-696 bp 1.0 79.6
clpP-psbB_p2 ACCCAAGTCGCTTCTTCATC CCAGCCCAACCAGAAACTAG 50.0 55.0 57.9 58.2 701-702 bp 1.0 79.3
clpP-psbB_p3 GTCGCTTCTTCATCTCCAGG CGATACCAAGGCAAACCCAT 55.0 50.0 58.1 58.2 611-612 bp 1.0 79.3
clpP-psbB_p4 TTCATCTCCAGGAATCCGGT CCAGCCCAACCAGAAACTAG 50.0 55.0 58.1 58.2 687-688 bp 1.0 79.2
clpP-psbB_p5 TCAACCCAAGTCGCTTCTTC CCAGCCCAACCAGAAACTAG 50.0 55.0 58.1 58.2 704-705 bp 1.0 79.1