Hotspot

petN-trnC-GCA

LSC · 17716-18648 · 933 bp

Back to Littledalea

Pi 0.000536

MarkerSeek score 45.10

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 933
variable_sites: 1
pi: 0.000536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	1041-1043 bp	1.0	70.7
petN-trnC-GCA_p2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	1040-1042 bp	1.0	70.7
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	982-984 bp	1.0	70.4
petN-trnC-GCA_p4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	1043-1045 bp	1.0	70.2
petN-trnC-GCA_p5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	1042-1044 bp	1.0	70.2