Hotspot

trnG-UCC-trnT-GGU

LSC · 13541-14780 · 1240 bp

Back to Littledalea

Pi 0.000806
MarkerSeek score 45.30
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1240
variable_sites
2
pi
0.000806
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1330 bp 1.0 40.9
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TGACTTACGTCTTAGCACGAG 38.1 47.6 55.5 57.5 1390 bp 1.0 40.1
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1326 bp 1.0 40.1
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1327 bp 1.0 40.1
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1332 bp 1.0 40.1