Hotspot

psbK-psbI

LSC · 6918-7331 · 414 bp

Back to Littledalea

Pi 0.00242

MarkerSeek score 42.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 2
pi: 0.00242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCGCGTCCAG	50.0	50.0	57.9	57.9	557-558 bp	1.0	79.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	50.0	50.0	57.9	57.8	535-536 bp	1.0	78.1
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	554-555 bp	1.0	77.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	555-556 bp	1.0	77.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	556-557 bp	1.0	77.4