Hotspot

psbA-trnK-UUU

LSC · 1153-1386 · 234 bp

Back to Littledalea

Pi 0

MarkerSeek score 21.80

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAAACACCGAACCATCCGAT	ACTAGTTCCGGGTTCGAGTC	45.0	55.0	57.8	58.8	373 bp	1.0	71.8
psbA-trnK-UUU_p2	AAAACACCGAACCATCCGAT	GACTAGTTCCGGGTTCGAGT	45.0	55.0	57.8	58.8	374 bp	1.0	71.8
psbA-trnK-UUU_p3	AAAACACCGAACCATCCGAT	ATCCGACTAGTTCCGGGTTC	45.0	55.0	57.8	58.9	378 bp	1.0	71.3
psbA-trnK-UUU_p4	GCTTGTACTTTCGCGTCTCT	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.3	58.8	302 bp	1.0	71.0
psbA-trnK-UUU_p5	GCTTGTACTTTCGCGTCTCT	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.3	58.8	303 bp	1.0	71.0