Hotspot

rpl32-trnL-UAG

SSC · 117386-118777 · 1392 bp

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Pi 0.0167
MarkerSeek score 69.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1392
variable_sites
128
pi
0.0167
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGACAAACAAGT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 55.2 57.9 1458-1482 bp 1.0 66.4
rpl32-trnL-UAG_p2 TTTTTGTGCGACAAACAAGT AGCAGCGTGTCTACCAATTT 35.0 45.0 55.2 57.8 1451-1475 bp 1.0 66.0
rpl32-trnL-UAG_p3 TTTTTGTGCGACAAACAAGT CCTAAGAGCAGCGTGTCTAC 35.0 55.0 55.2 57.8 1457-1481 bp 1.0 65.9
rpl32-trnL-UAG_p4 TTTTTGTGCGACAAACAAGT CTAAGAGCAGCGTGTCTACC 35.0 55.0 55.2 57.8 1456-1480 bp 1.0 65.9
rpl32-trnL-UAG_p5 TTTTTGTGCGACAAACAAGT GAGCAGCGTGTCTACCAATT 35.0 50.0 55.2 58.3 1452-1476 bp 1.0 65.3