Hotspot

ycf4-cemA

LSC · 63618-64531 · 914 bp

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Pi 0.00851
MarkerSeek score 59.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
914
variable_sites
55
pi
0.00851
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 AAAAAGCTGCTGAATTGGCC AGATCCACCAGGGCAAAAAG 45.0 50.0 57.8 58.1 1026-1049 bp 1.0 84.9
ycf4-cemA_p2 AAAAAGCTGCTGAATTGGCC GATCCACCAGGGCAAAAAGA 45.0 50.0 57.8 58.1 1025-1048 bp 1.0 84.9
ycf4-cemA_p3 AAAAAGCTGCTGAATTGGCC GAGATCCACCAGGGCAAAAA 45.0 50.0 57.8 58.1 1027-1050 bp 1.0 84.8
ycf4-cemA_p4 AAAGCTGCTGAATTGGCCTA AGATCCACCAGGGCAAAAAG 45.0 50.0 57.8 58.1 1024-1047 bp 1.0 84.5
ycf4-cemA_p5 AAAGCTGCTGAATTGGCCTA GATCCACCAGGGCAAAAAGA 45.0 50.0 57.8 58.1 1023-1046 bp 1.0 84.5