Hotspot

lhbA-trnG-GCC

LSC · 38471-38745 · 275 bp

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Pi 0.0116
MarkerSeek score 59.80
Primer available yes
Label lhbA-trnG-GCC

Feature Metadata

label_name
lhbA-trnG-GCC
feature_type
igs
region
LSC
length_bp
275
variable_sites
17
pi
0.0116
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
lhbA-trnG-GCC_p1 TGCTTCTTCTGATGGTTGGT CCTTACTTTACCATGGGCGG 45.0 55.0 57.0 58.3 442-506 bp 1.0 76.8
lhbA-trnG-GCC_p2 TGCTTCTTCTGATGGTTGGT ACCTTACTTTACCATGGGCG 45.0 50.0 57.0 57.6 443-507 bp 1.0 76.1
lhbA-trnG-GCC_p3 TGCTTCTTCTGATGGTTGGT GATCGAACCCGCATCTTCTC 45.0 55.0 57.0 58.5 414-478 bp 1.0 75.5
lhbA-trnG-GCC_p4 GCTTCTTCTGATGGTTGGTCA CCTTACTTTACCATGGGCGG 47.6 55.0 58.2 58.3 441-505 bp 1.0 74.7
lhbA-trnG-GCC_p5 TGCTTCTTCTGATGGTTGGTC CCTTACTTTACCATGGGCGG 47.6 55.0 58.2 58.3 442-506 bp 1.0 74.7